Search results for " 30"
showing 10 items of 203 documents
Changes in lipid and carotenoid metabolism in Chlamydomonas reinhardtii during induction of CO2-concentrating mechanism: Cellular response to low CO2…
2020
Photosynthetic organisms strictly depend on CO2 availability and the CO2:O2 ratio, as both CO2/O2 compete for catalytic site of Rubisco. Green alga Chlamydomonas reinhardtii, can overcome CO2 shortage by inducing CO2-concentrating mechanism (CCM). Cells transferred to low-CO2 are subjected to light-driven oxidative stress due to decrease in the electron sink. Response to environmental perturbations is mediated to some extent by changes in the lipid and carotenoid metabolism. We thus hypothesize that when cells are challenged with changes in CO2 availability, changes in the lipidome and carotenoids profile occur. These changes expected to be transient, when CCM is activated, CO2 limitation w…
Degradation of sexual reproduction in Veronica filiformis after introduction to Europe
2011
Abstract Background Baker’s law predicts that self-incompatible plant species are generally poor colonizers because their mating system requires a high diversity of genetically differentiated individuals and thus self-compatibility should develop after long-distance dispersal. However, cases like the introduction of the self-incompatible Veronica filiformis (Plantaginaceae) to Europe constitute an often overlooked alternative to this rule. This species was introduced from subalpine areas of the Pontic-Caucasian Mountains and colonized many parts of Central and Western Europe in the last century, apparently without producing seeds. To investigate the consequences of the absence of sexual rep…
Ultrasonic Welding of PBT-GF30 (70% Polybutylene Terephthalate + 30% Fiber Glass) and Expanded Polytetrafluoroethylene (e-PTFE)
2021
The ultrasonic welding of polymeric materials is one of the methods often used in practice. However, each couple of material subjected to ultrasonic welding is characterized by different values of technological parameters. Therefore, the main objective of the research presented in this paper is to optimize the parameters for the ultrasonic welding of two materials, namely PBT-GF30 (70% polybutylene terephthalate + 30% fiber glass) and expanded polytetrafluoroethylene (e-PTFE). In this sense, the research was carried out considering a plate-type part made of PBT-GF30, which had a thickness of 2.1 mm, and a membrane-type part made of e-PTFE, with a thickness of 0.3 mm. The condition imposed o…
El sentimiento de superioridad de los jesuitas tras la extinción de la Compañía de Jesús
2020
La portada de las Constitutiones Fratrum Ordinis ... Mariae de Monte Carmeli, editada por Pedro Patricio Mey en 1590, y su influencia
2011
2,3-Dihydrobenzofuran privileged structures as new bioinspired lead compounds for the design of mPGES-1 inhibitors
2016
International audience; 2,3-Dihydrobenzofurans are proposed as privileged structures and used as chemical platform to design small compound libraries. By combining molecular docking calculations and experimental verification of biochemical interference, we selected some potential inhibitors of microsomal prostaglandin E2 synthase (mPGES)-1. Starting from low affinity natural product 1, by our combined approach we identified the compounds 19 and 20 with biological activity in the low micromolar range. Our data suggest that the 2,3-dihydrobenzofuran derivatives might be suitable bioinspired lead compounds for development of new generation mPGES-1 inhibitors with increased affinity.
Antitumor effect of oncolytic virus and paclitaxel encapsulated in extracellular vesicles for lung cancer treatment
2018
Standard of care for cancer is commonly a combination of surgery with radiotherapy or chemoradiotherapy. However, in some advanced cancer patients this approach might still remaininefficient and may cause many side effects, including severe complications and even death. Oncolytic viruses exhibit different anti-cancer mechanisms compared with conventional therapies, allowing the possibility for improved effect in cancer therapy. Chemotherapeutics combined with oncolytic viruses exhibit stronger cytotoxic responses and oncolysis. Here, we have investigated the systemic delivery of the oncolytic adenovirus and paclitaxel encapsulated in extracellular vesicles (EV) formulation that, in vitro, s…
Engineering approaches in siRNA delivery.
2017
siRNAs are very potent drug molecules, able to silence genes involved in pathologies development. siRNAs have virtually an unlimited therapeutic potential, particularly for the treatment of inflammatory diseases. However, their use in clinical practice is limited because of their unfavorable properties to interact and not to degrade in physiological environments. In particular they are large macromolecules, negatively charged, which undergo rapid degradation by plasmatic enzymes, are subject to fast renal clearance/hepatic sequestration, and can hardly cross cellular membranes. These aspects seriously impair siRNAs as therapeutics. As in all the other fields of science, siRNAs management ca…
Tetraspanin CD63 controls basolateral sorting of organic cation transporter 2 in renal proximal tubules.
2016
CD63 is a ubiquitously expressed member of the tetraspanin superfamily. Using a mating-based split-ubiquitin-yeast 2-hybrid system, pull-down experiments, total internal reflection fluorescence microscopy, Forster resonance energy transfer, and biotinylation assays, we found that CD63 interacts with human organic cation transporter 2 (hOCT2), which transports endogenous and exogenous substrates, such as neurotransmitters and drugs in several epithelial cells. CD63 overexpression affects cellular localization of hOCT2 expressed in human embryonic kidney (HEK)293 cells. Studies with CD63-knockout mice indicate that in renal proximal tubules, CD63 determines the insertion of the mouse ortholog…
Complete Genome Sequence of KPC-3- and CTX-M-15-Producing Klebsiella pneumoniae Sequence Type 307.
2016
ABSTRACT Klebsiella pneumoniae sequence type (ST) 307, carrying bla KPC-3 , bla CTX-M-15 , bla OXA-1 , aac(6′)-Ib-cr , and qnrB 1 genes, is replacing the predominant hyperepidemic ST258 clone in Italy. Whole-genome and complete plasmid sequencing of one ST307 strain was performed and new features were identified.